What types of samples can be processed for EV isolation?

We routinely process cell culture conditioned medium, human plasma, urine, and tissue homogenates. Other biological fluids such as saliva or cerebrospinal fluid can be handled after a feasibility discussion. The optimal isolation method is selected based on sample viscosity, volume, and desired EV subtype.

How do you ensure the purity of isolated EVs?

Purity is assessed by multiple orthogonal methods: particle/protein ratio, absence of common co‑isolated contaminants (apolipoproteins, albumin) by Western blot, and single‑particle phenotyping where possible. We adhere to MISEV guidelines for reporting and provide a purity index for every batch.

Can you isolate different EV subpopulations from the same sample?

Yes, we offer option to perform density gradient separation to enrich large EVs, small EVs, and non‑vesicular fractions (exomeres, supermeres) from a single starting material. The number of fractions and the resolution are scoped per project to match your research question.

What characterization techniques are included in the standard service?

Our standard panel includes nanoparticle tracking analysis (NTA) for size/concentration, flow cytometry for at least three tetraspanin markers (CD9, CD63, CD81), Western blot for marker enrichment and contamination check, and transmission electron microscopy for morphology. Additional techniques (proteomics, RNA profiling, functional assays) can be added as custom modules.

Is the service suitable for clinical or diagnostic purposes?

No, this service is intended for research use only. It is not designed or validated for clinical diagnostic or therapeutic applications. For preclinical research, we can implement additional QC steps but the workflow remains research‑grade.

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EV Isolation, Purification & Characterization Service

Extracellular Vesicle Services

We provide integrated EV isolation and purification from diverse biological sources, coupled with rigorous characterization to support your downstream applications.

Sample-optimized isolation methods for cell culture, plasma, urine, and tissues
Multi-technique characterization: NTA, flow cytometry, Western blot, electron microscopy
High-purity EV fractions with detailed yield and purity assessments
Customizable workflow to accommodate specific downstream needs
Comprehensive documentation for publication-ready reporting

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Why Choose a Standardized EV Service?

Achieve reproducible, well-characterized extracellular vesicles for biomarker discovery, therapeutic development, and mechanistic studies. Each preparation is documented with detailed QC metrics to support confident interpretation of downstream results.

Reproducible protocols reduce batch-to-batch variability
Flexible method selection tailored to your sample type and research goals
In-depth QC metrics including particle/protein ratio and surface marker confirmation
Expert guidance from sample preparation through data interpretation

Reproducible

Comprehensive

Customized

How It Works

Integrated EV Isolation & Characterization

Our approach combines established physical separation techniques with state-of-the-art analytical tools to deliver pure, well-defined EV preparations.

Isolation Pipeline

We employ a sequence of clarification, concentration, and purification steps to isolate EVs from your starting material.

Low-speed centrifugation to remove cells and large debris
Ultracentrifugation or tangential flow filtration for particle concentration
Size exclusion chromatography to separate EVs from soluble proteins
Optional density gradient separation for subpopulation enrichment

Multi-Characterization

Purified EVs are characterized by orthogonal techniques to assess size, concentration, purity, and marker expression.

Nanoparticle tracking analysis for particle size distribution and concentration
Flow cytometry for surface marker phenotyping (e.g., CD9, CD63, CD81)
Western blot detection of EV-enriched and contaminant proteins
Electron microscopy for morphology and integrity verification

Quality Control

Every batch is evaluated for particle/protein ratio, marker enrichment, and absence of non-EV contaminants according to MISEV guidelines.

Statistical Rigor

Characterization assays are performed in replicates where feasible, and data are reported with confidence intervals to ensure reliability.

Biological Context

We help connect EV properties to functional outcomes, supporting downstream applications like functional assays or cargo analysis.

What You Receive

Comprehensive EV Service Deliverables

From raw data to interpretable reports, every project delivers the key metrics needed for publication and further studies.

Data Deliverables

Particle concentration and size distribution (NTA histograms)
Purity index: particle/protein ratio and protein marker blots
Flow cytometry data with MFI of EV markers
Electron microscopy images and representative fields

Reporting & Support

Detailed methods section written for submission
QC summary with assessment of purity and yield
Consultation for optimization or additional downstream analyses
Raw data files in standard formats (CSV, PDF, TIFF)

Service Scope

Customizable Parameters for Your Project

Every project is scoped individually after consultation to match your sample type, volume, and research questions.

Parameter	Typical Project Scope
Sample Type	Cell culture medium, human plasma, urine, tissue homogenates, or other biological fluids
Isolation Methods	Ultracentrifugation, size exclusion chromatography (SEC), tangential flow filtration (TFF), and density gradient separation are applied individually or in combination based on sample characteristics and research objectives.
Characterization Techniques	NTA, flow cytometry (≥3 EV markers), Western blot, transmission electron microscopy, optionally proteomics/RNA analysis
Purity Assessment	Particle/protein ratio, lipoprotein/contaminant markers, single-particle phenotyping where feasible
Number of EV Fractions	Single bulk EV preparation, or up to six subpopulations (large EVs, small EVs, non‑vesicular fractions) as scoped
Downstream Compatibility	EVs suitable for functional assays, cargo extraction (RNA/protein), or biobanking; buffers can be adjusted per request
QC Metrics Provided	Particle yield, size mode, purity index, presence of tetraspanin markers, absence of cellular contaminants
Documentation	Full methods description, QC report, raw data files, and a summary of results for publication support

Project Workflow

From Sample to Characterization

Our five‑stage pipeline ensures that each EV preparation is processed consistently and thoroughly analyzed.

Sample Collection & Clarification

We receive your sample and perform low‑speed centrifugation to remove cells and large debris, followed by filtration if needed to eliminate aggregates.

Concentration & Purification

EVs are concentrated by ultracentrifugation or tangential flow filtration. Size exclusion chromatography then separates vesicles from soluble proteins and smaller contaminants.

Optional Density Gradient Separation

For projects requiring subpopulation enrichment, we apply an iodixanol or sucrose density gradient to separate large EVs, small EVs, and non‑vesicular fractions.

Multi‑Modal Characterization

Purified fractions are analyzed by nanoparticle tracking analysis, flow cytometry for surface markers, Western blot for protein cargo, and electron microscopy for morphology.

Data Analysis & Reporting

We compile all QC data, produce particle size histograms, marker expression plots, and a detailed methods section. A consultation is included to review results and plan next steps.

Applications

Tailored for EV Research Needs

Our service supports a wide range of EV studies, from basic biology to translational projects.

Identify Circulating EV Biomarkers

Well‑characterized EV preparations from plasma or urine enable unbiased screening of protein and RNA cargo for disease‐specific signatures.

High purity reduces false positives from contaminating proteins
Compatible with proteomics and RNA‑seq downstream
Reproducible isolation across cohorts

Produce EVs for Drug Delivery Studies

Our isolation workflow yields intact, functional EVs suitable for loading, formulation, and preclinical testing.

Preservation of membrane integrity confirmed by EM and functional assays
Scalable from research to pilot batches using TFF
Optional sterile filtration for in vivo work

Dissect EV Subpopulations for Signaling Research

Density gradient separation allows you to assign cargo and functions to specific EV subtypes or non‑vesicular fractions.

Up to six distinct fractions per starting material
Each fraction characterized for size, marker, and cargo composition
Directly applicable to uptake and functional assays

Frequently Asked Questions

Common Questions About EV Isolation Services

Get answers to typical concerns regarding sample compatibility, purity, and customization.

We routinely process cell culture conditioned medium, human plasma, urine, and tissue homogenates. Other biological fluids such as saliva or cerebrospinal fluid can be handled after a feasibility discussion. The optimal isolation method is selected based on sample viscosity, volume, and desired EV subtype.
Purity is assessed by multiple orthogonal methods: particle/protein ratio, absence of common co‑isolated contaminants (apolipoproteins, albumin) by Western blot, and single‑particle phenotyping where possible. We adhere to MISEV guidelines for reporting and provide a purity index for every batch.
Yes, we offer option to perform density gradient separation to enrich large EVs, small EVs, and non‑vesicular fractions (exomeres, supermeres) from a single starting material. The number of fractions and the resolution are scoped per project to match your research question.
Our standard panel includes nanoparticle tracking analysis (NTA) for size/concentration, flow cytometry for at least three tetraspanin markers (CD9, CD63, CD81), Western blot for marker enrichment and contamination check, and transmission electron microscopy for morphology. Additional techniques (proteomics, RNA profiling, functional assays) can be added as custom modules.
No, this service is intended for research use only. It is not designed or validated for clinical diagnostic or therapeutic applications. For preclinical research, we can implement additional QC steps but the workflow remains research‑grade.

Scientific Literature

References

Crescitelli R, Lässer C, Lötvall J. Isolation and characterization of extracellular vesicle subpopulations from tissues. Nature protocols. 2021;16(3):1548-1580. View on PubMed
Zhang Q, Jeppesen DK, Higginbotham JN, et al. Comprehensive isolation of extracellular vesicles and nanoparticles. Nature protocols. 2023;18(5):1462-1487. View on PubMed
Li B, Kalluri VS, Weaver AM, et al. Advancements in extracellular vesicle research. Extracellular vesicle. 2026;7. View on PubMed

Please kindly note that our products and services are for research use only.