Bioinformatics Analysis Service
Bioinformatics Analysis Service
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Bioinformatics Analysis Service

Bioinformatics Analysis

Transform raw RNA-seq data into biological insight with expert bioinformatics support tailored to your project goals.

  • Full transcriptome pipeline: quality control, alignment, quantification, and differential expression
  • Multi-omics integration and custom statistical modeling for complex study designs
  • Publication-ready figures, tables, and comprehensive reporting
  • Flexible scope: from standalone analysis to end-to-end project support
  • Dedicated bioinformatician consultation for data interpretation

Why Outsource Transcriptome Bioinformatics?

Research projects stall without computational expertise—our team accelerates your findings with rigorous, reproducible analysis.

  • Customized analysis plans aligned with your experimental design
  • Transparent workflows and full data ownership retained by your group
  • Expert support for grant writing and publication review
Reproducible
Customizable
Publication-ready
Our Approach

End-to-End Bioinformatics Pipeline

We integrate robust computational methods with biological domain knowledge to deliver meaningful results from your transcriptome data.

Core Transcriptome Pipeline

Standardized yet flexible workflow processing raw FASTQ reads through to annotated expression tables and differential analysis.

  • Adapter trimming and quality assessment with FastQC/MultiQC
  • Splice-aware alignment (STAR or HISAT2) to reference genome
  • Gene/transcript quantification (featureCounts, Salmon, RSEM)

Multi-Omics Integration

Combine transcriptomic data with other modalities (genomics, epigenomics, proteomics) for deeper biological insight.

  • Correlation analysis between RNA expression and CNV/methylation
  • Pathway and network enrichment across omics layers
  • Custom integration strategies for exploratory discovery

Quality Control

Rigorous assessment at every step—raw data, alignment, count matrices—with clear metrics and outlier identification.

Statistical Rigor

State-of-the-art tools (DESeq2, edgeR, limma) with appropriate multiple-testing correction (BH) and batch effect adjustment (ComBat).

Biological Interpretation

Functional annotation, GSEA, immune deconvolution, and splice variant analysis to connect expression changes to phenotype.

Deliverables

What You Receive

Clear, actionable results designed for publication and follow-up experiments.

Data Deliverables

  • Processed count matrices (gene-level and transcript-level)
  • Normalized expression tables (TPM/FPKM)
  • Alignment files (BAM) and quality reports
  • Variant calls (SNP/INDEL) from RNA-seq if scoped

Reporting & Support

  • Comprehensive analysis report with methods, figures, and tables
  • Custom visualizations (heatmaps, volcano plots, PCA)
  • Post-delivery interpretation consultation
  • Full documentation for reproducibility
Service Scope

Customizable Analysis Parameters

Each project is scoped individually—no fixed packages. Typical parameters include:

ParameterTypical Project Scope
Input data typeFASTQ from Illumina, Ion Torrent, or PacBio (scoped per project)
Quality controlFastQC, MultiQC, adapter trimming (Trimmomatic, Cutadapt)
Alignment toolSTAR, HISAT2, or Salmon (selectable)
QuantificationfeatureCounts, RSEM, Salmon – gene/transcript level
Differential expressionDESeq2, edgeR, limma-voom – with batch correction (ComBat)
Alternative splicingrMATS, SUPPA (if scoped)
Functional analysisGSEA, GO/KEGG enrichment, immune deconvolution
Multi-omics integrationCorrelation, network, integrative clustering (scoped per project)
Custom scripts/pipelinesR, Python, Bash – as agreed
Reporting formatPDF report with figures + supplementary data files
Workflow

How We Deliver Your Analysis

A transparent, stepwise process from data receipt to final report.

Project Scoping & Data Transfer

We discuss your experimental design and analysis needs, then securely receive raw FASTQ files or sequencing data from your preferred platform.

Quality Control & Preprocessing

Read quality is assessed with FastQC; adapters and low-quality bases are trimmed using Trimmomatic or Cutadapt to ensure clean input for alignment.

Alignment & Quantification

Cleaned reads are aligned to the reference genome with STAR or HISAT2; gene/transcript counts are generated with featureCounts, Salmon, or RSEM.

Statistical Analysis & Visualization

Differential expression is performed with DESeq2 or edgeR, including batch correction when needed. Results are visualized in PCA plots, heatmaps, and volcano plots.

Interpretation & Reporting

We deliver a comprehensive report with functional enrichment, pathways, and custom figures. A follow-up call is provided to discuss findings and next steps.

Applications

Research Contexts We Support

Our transcriptome bioinformatics service is applied across diverse biological and biomedical studies.

Identify Tumor Biomarkers and Mechanisms

From differential expression to immune microenvironment analysis, we help uncover oncogenic drivers and therapeutic targets across cancer types.

  • Pan-cancer transcriptomic comparisons and survival analysis
  • Immune infiltration and checkpoint expression profiling
  • Splice variant and fusion gene detection

Elucidate Transcriptional Signatures in Small Cohorts

Our tailored pipelines handle low sample numbers and can integrate with family-based exome data to prioritize candidate genes.

  • Differential expression in limited sample sets
  • Pathway perturbation analysis and biomarker discovery
  • Integration with variant data for diagnostic insights

Characterize Gene Expression Dynamics

Time-series and developmental stage comparisons are supported with appropriate modeling and trajectory analysis.

  • Temporal expression profiling and clustering
  • Pseudotime analysis from bulk or single-cell data
  • Enrichment of developmental pathways
FAQ

Common Questions About Our Bioinformatics Service

We address key concerns to help you decide if our service is right for your project.

  • We primarily analyze bulk RNA-seq data (single-end or paired-end) from Illumina platforms. We can also work with Ion Torrent, PacBio Iso-Seq, and targeted transcriptome data upon scoping. Please contact us with your specific platform and data volume to confirm feasibility.

  • Yes. Our services are fully customizable. We design analysis plans based on your hypothesis, sample size, and desired endpoints. Examples include specialized network analysis, machine learning classifiers, or integrative multi-omics pipelines. The scope is agreed during the initial consultation.

  • We treat all client data as confidential. Analysis is conducted on secure infrastructure, and you retain full ownership of both raw and processed data. We do not claim authorship or intellectual property rights. Data sharing and publication guidelines are defined in the service agreement.

  • Yes. Many clients use our analysis results for grant submissions and publications. We provide detailed methods sections and can assist with figure preparation and statistical descriptions. Our team is experienced in writing bioinformatics sections for diverse funding agencies.

  • Our bioinformaticians have extensive experience with pan-cancer datasets (TCGA, GTEx, CPTAC) and have published on biomarker discovery and immune microenvironment analysis. We apply similar rigorous pipelines to your specific project data.

  • Batch effects are identified using PCA and corrected with tools like ComBat or limma's removeBatchEffect. We also incorporate covariates (e.g., age, sex, processing batch) into statistical models as needed. The approach is tailored to your study design.

  • While there is no strict minimum, statistical power increases with sample size. We can analyze as few as 2–3 replicates per condition, but results should be interpreted cautiously. For small datasets, we offer specialized pipelines with moderated statistics (e.g., limma) to improve reliability.

Scientific Literature

References

  1. Liang X, Li L, Fan Y. Diagnostic, Prognostic, and Immunological Roles of HELLS in Pan-Cancer: A Bioinformatics Analysis. Frontiers in immunology. 2022;13:870726. View on PubMed
  2. Xiao K, Ullah I, Yang F, et al. Comprehensive bioinformatics analysis of FXR1 across pan-cancer: Unraveling its diagnostic, prognostic, and immunological significance. Medicine. 2023;102(48):e36456. View on PubMed
  3. Anderson R. Basics of Bash. Methods in molecular biology (Clifton, N.J.). 2022;2443:161-180. View on PubMed
  4. Facchiano F, D'Arcangelo D, Facchiano A. Alpha-2-Macroglobulin Is a Novel Anticancer Agent. Oncology. 2024;102(7):641-645. View on PubMed
  5. Hassan MU, Williamson MP. Bioinformatic analysis of WxL domain proteins. Saudi journal of biological sciences. 2023;30(2):103526. View on PubMed

Please kindly note that our products and services are for research use only.